Journal: Toxins

Article Title: Co-Expression and Co-Purification Enable Manufacturing of a Six-Monoclonal Antibody Botulinum Antitoxin Cocktail

doi: 10.3390/toxins18050199

Figure Lengend Snippet: IEX HPLC Analysis of 5 mL HiTrap Capto S Column Fraction 15 (blue) and S column load (black). The inset shows the protein concentration measured by A280 on the Nanodrop spectrophotometer for the collected peak fractions (1 mL per fraction). Fraction 15 in the histogram is highlighted in blue. (IEX-HPLC of individual purified antibodies is shown in .) The column was loaded with 20 μg antibody.

Article Snippet: Cytiva HiTrap Capto MMC, Cytiva MabSelect PrismA, Cytiva Fibro PrismA, Eshmuno CP-FT, Cytiva Capto Q (1 mL), Cytiva Capto S (1 mL), Cytiva Capto Adhere (1 mL), MabSelect SuRe resin, MilliporeSigma ViResolve Modus 1.1 viral reduction filter (Sigma, St. Louis, MO, USA), Pall (Port Washington, NY, USA) Cadence Omega 0.1 m 2 30 kDa TFF cassette.

Techniques: Protein Concentration, Spectrophotometry, Purification